The service, which is expected to launch in January, will offer 3,500 hours of programming from AOL-owned Warner Bros library including Lois & Clark: the New Adventures of Superman, Wonder Woman and Babylon 5.
In2TV will be based around six genre-based channels including comedy, drama, animation, and action/adventure.
AOL Media Networks executive vice president Kevin Conroy said: "We want to create a new broadband network for content looking for its next window of distribution. This is a television service that is available whenever, wherever in the digital home."
The move is the latest development from US content owners who are keen to develop markets for online viewing. Last week NBC Universal and CBS announced a deal to make primetime programming available on-demand just hours after broadcast, while Walt Disney has said it would make ABC hits such Lost and Desperate Housewives available to view on iPods.